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. 2016 Sep 11;(115):54243. doi: 10.3791/54243
Step Number Treatment Purpose Time (hr)
1 70% IMS Dehydration 3
2 90% IMS Dehydration 2.5
3 95% IMS Dehydration 1.5
4 100% IMS Dehydration 1.5
5 100% IMS Dehydration 1.5
6 100% IMS Dehydration 1.5
7 100% IMS Dehydration 1.5
8 Histology clearing agent Clearing 1.5
9 Histology clearing agent Clearing 1.5
10 Histology clearing agent Clearing 1.5
11 WAX Wax infiltration 1.25
12 WAX Wax infiltration 1.25
20 hr TOTAL