Step Number | Treatment | Purpose | Time (hr) |
1 | 70% IMS | Dehydration | 3 |
2 | 90% IMS | Dehydration | 2.5 |
3 | 95% IMS | Dehydration | 1.5 |
4 | 100% IMS | Dehydration | 1.5 |
5 | 100% IMS | Dehydration | 1.5 |
6 | 100% IMS | Dehydration | 1.5 |
7 | 100% IMS | Dehydration | 1.5 |
8 | Histology clearing agent | Clearing | 1.5 |
9 | Histology clearing agent | Clearing | 1.5 |
10 | Histology clearing agent | Clearing | 1.5 |
11 | WAX | Wax infiltration | 1.25 |
12 | WAX | Wax infiltration | 1.25 |
20 hr TOTAL |