Stain no.	Stain	Purpose	Time (min)
1	Histology Clearing agent	Dissolves wax	15
2	100% IMS	Hydration	2
3	90% IMS	Hydration	2
4	70% IMS	Hydration	2
5	TAP WATER (RUNNING)	Rinse	2
6	HAEMOTOXYLIN	Stains cell nuclei blue	10
7	TAP WATER (RUNNING)	Remove excess	10
8	Acidified IMS	Dechlorination	20 sec
9	TAP WATER (RUNNING)	Rinse	20 sec
10	LiCO3	Salt	20 sec
11	TAP WATER (RUNNING)	Rinse	20 sec
12	1% EOSIN (AQUEOUS)	Stains cytoplasm pink	20 sec
13	TAP WATER (RUNNING)	Remove excess	5
14	70% IMS	Dehydration	2
15	90% IMS	Dehydration	2
16	100% IMS	Dehydration	5
17	Histology Clearing agent	Remove IMS, binding agent	5