Column: | PL gel, 50 A, 300 x 7.5 mm, 5 µm |
Guard Column: | PL gel, 50 x 7.5 mm, 5 µm |
Mobile phase: | Dichloromethane |
Flow rate: | 1 ml per min |
Column temperature: | 25 °C |
UV detector: | 210 nm |
Injection volume: | 95 µl |
Injection mode: | Standard |
Draw speed: | 500 ml per min |
Eject speed: | 500 ml per min |
Draw position: | 3 mm |
Fraction collected: | 3 ml fraction (7 - 10 min) in 10 ml vials |