| Amine Reaction | Thiol Reaction | |||||
| Well | Isotype | Antibody Recovery (µg) | Dye to Antibody Ratio | Antibody Recovery (µg) | Dye to Antibody Ratio | |
| 1 | mouse IgG1 | Purification | 54.8 ± 1.8 | 0 | 49.2 ± 2.0 | 0 |
| 2 | Conjugation | 27.4 ± 3.2 | 4.2 ± 0.2 | 46.2 ± 3.5 | 1.6 ± 0.2 | |
| 3 | mouse IgG2A | Purification | 85.9 ± 6.4 | 0 | 75.3 ± 8.0 | 0 |
| 4 | Conjugation | 60.7 ± 3.2 | 4.4 ± 0.1 | 61.5 ± 7.4 | 5.5 ± 0.4 | |
| 5 | mouse IgG2B | Purification | 79.9 ± 6.9 | 0 | 73.3 ± 1.4 | 0 |
| 6 | Conjugation | 66.7 ± 0.5 | 5.6 ± 0.1 | 55.7 ± 0.9 | 5.2 ± 0.5 |
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