Table 1. Characteristics of tumors and their amplicons.
| Characteristic | Case 4 | Case 7 | Case 14 | Case 21 | Case 22 | Case 30 | Case 34 |
|---|---|---|---|---|---|---|---|
| Histology* | ODA | GBM | ODA | GBM | ODA | GBM | GBM |
| Material† | N/F/X | X | N/F/X | F/X | F/X | F | F |
| Chromosomes‡ | 90 | 45 | 47 | 70 | 47 | 91 | 47 |
| Chromosomes 7§ | 6 | 3 | 3 | 3 | 3 | 6 | — |
| Amplification¶ | |||||||
| Xenograft | 9 (7-13) | 8 (5-12) | 17 (12-24) | 32 (22-45) | 55 (40-70) | — | — |
| Fresh | 10 (7-14) | — | 17 (13-25) | 30 (20-45) | 60 (55-80) | 30 (20-40) | 63 (50-80) |
| Amplicon∥ | |||||||
| 5′ Breakpoint | 54,492,001 | 54,168,756 | 47,078,805 | 53,489,375 | 54,349,769 | 53,426,589 | 54,440,318 |
| 3′ Breakpoint | 56,100,888 | 55,697,845 | 55,057,216 | 55,121,943 | 55,056,001 | 55,562,319 | 55,641,516 |
| Length, bp | 1,608,892 | 1,529,091 | 941,200 | 1,530,714 | 706,234 | 2,099,732 | 1,201,200 |
| Deletion∥ | |||||||
| 5′ Breakpoint | — | — | 47,118,693 | 54,862,131 | — | — | — |
| 3′ Breakpoint | — | — | 54,155,906 | 54,963,991 | — | — | — |
| Length, bp | — | — | 7,037,214 | 101,865 | — | — | — |
ODA, oligodendroglioma; GBM, glioblastoma multiforme; N, normal tissue; F, fresh tumor; X, xenografted tumor.
Histological types.
Available biological material.
Modal chromosome number estimated from analysis of R-banded metaphase chromosomes.
Number of chromosomes 7 determined by FISH with chromosome 7 painting probes.
Mean level of amplification. Experimental ranges are given in parentheses.
Localization of the breakpoints that generated the amplicons and their internal deletions. Breakpoints are arbitrarily located at the first base found outside of the region of microhomology (see Fig. 3). Positions are indicated according to the human genome sequence published on the University of California, Santa Cruz (UCSC) Genome Bioinformatics web site (release date July 2003).