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. 2004 Jul 22;101(31):11374–11379. doi: 10.1073/pnas.0404318101

Fig. 3.

Fig. 3.

Colocalization of two key kinetochore proteins CaCse4p and CaMif2p to a 3-kb region of Can. albicans chromosome 7. A standard ChIP assay was performed on strains CAI4, SC5314, and CAMB1 with primer pairs (Table 3) that amplify 178- to 292-bp regions spaced approximately every 1 kb between Orf19.6522.prot and Orf19.6524.prot. PCR with serial dilutions of total DNA and with or without antibody ChIP DNA fractions were performed. (Left) The ethidium bromide-stained PCR products obtained from budding CAI4 cells are shown here as negative images and are aligned with a scale bar to show the locations tested for enrichment. (Right) Enrichment of CaCse4p binding on chromosome 7 in budded CAI4 cells (black bars) and hyphal SC5314 cells (hatched bars) and enrichment of CaMif2p binding on chromosome 7 in budded CAMB1 cells (speckled bars) as determined by standard ChIP assays are graphically represented. Enrichment equals (+Ab) minus (–Ab) signals divided by the total DNA signal and is normalized to a value of 1 for CaLEU2.