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. 2004 Jul 22;101(31):11374–11379. doi: 10.1073/pnas.0404318101

Fig. 4.

Fig. 4.

CaMif2p, the CENP-C homolog in Can. albicans, is an essential protein. (a) CaMif2p contains a region that is conserved in proteins of the CENP-C family. Conserved regions of various organisms were identified by the Blockmaker server (www.blocks.fhcrc.org). Black and gray boxes indicate identical and similar amino acid residues, respectively. GenBank accession numbers: Saccharomyces cerevisiae ScMif2p, Z28089; Schizosaccharomyces pombe SpCENP-C, CAB52737; C. elegans CeHCP4, AF321299; Mus musculus MmCENP-A, U03113; Gallus gallus GgCENP-A, AB004649; Ovis aries OaCENP-A, P49453; Homo sapiens HsCENP-A, M95724; and Arabidopsis thaliana AtCENP-A, AAF71990. (b) Structural schematic illustration of diploid CaMIF2 loci in strains CAMB1 and CAMB2. CAMB1 carries two CaMIF2 genes: one under its native promoter and a myc-tagged version under CaPCK1 promoter. CAMB2 is the same as CAMB1, except the CaMIF2 gene under its native promoter is disrupted by the CaHIS1 gene. (c) Shutdown of CaMif2p expression on glucose medium (Glu) prevents CAMB2 cell growth; induction of CaMif2p expression on succinate medium (Suc) allows CAMB2 cell growth. CAMB1 cells, carrying one intact CaMIF2 allele, grow on both media. Plates were incubated at 30°C for 5 days.