Skip to main content
. 2004 Jul 23;101(31):11392–11397. doi: 10.1073/pnas.0404382101

Fig. 5.

Fig. 5.

SpA treatment results in functional impairment of TI anti-TNP Ab responses from VH-targeted B cells. Groups of age-matched C57BL/6 mice received control or SpA treatment and after 2 weeks received immunizations with either TNP-LPS (TI-1) (A and B) or TNP-ficoll (TI-2) (C and D) immunogens. Two weeks after immunization, Ab responses were evaluated based on detection with tagged anti-IgM reagent (A and C) or tagged mSpA (B and D), which detects Fab-mediated SpA binding reactivity (12). Values in relative units were derived by interpolation from a standard curve of an IgM anti-TNP control. Pre- and postimmune anti-TNP values from individual mice are displayed after control (♦) or SpA treatment (○). Each group contained six or eight mice, as indicated. Statistical significance was determined for pre- and postimmune responses of a group based on the two-tailed paired Student t test, whereas other comparisons used the unpaired t test. P values are represented by *, <0.05; **, <0.01; ***, < 0.005; and ****, <0.005.