Table 1. Impaired granulopoiesis in G-CSF-/- mice during acute inflammatory arthritis.
| Day 0
|
Day 3
|
Day 7
|
||||
|---|---|---|---|---|---|---|
| B6 | G-CSF-/- | B6 | G-CSF-/- | B6 | G-CSF-/- | |
| Blood (10-4/ml) | ||||||
| Neutrophils | 33 ± 6 | 19 ± 3 | 40 ± 5 | 12 ± 3* | 63 ± 11 | 20 ± 4* |
| Monocytes | 4 ± 2 | 3 ± 1 | 2 ± 0 | 3 ± 1 | 6 ± 2 | 3 ± 0 |
| Bone marrow | ||||||
| Cells (10-6/femur) | 21.3 ± 1.0 | 19.0 ± 4.0 | 14.6 ± 0.8 | 12.2 ± 1.0 | 17.1 ± 2.3 | 14.6 ± 1.5 |
| Blasts, % | 3.5 ± 0.5 | 1.5 ± 0.5 | 1.6 ± 0.7 | 3.8 ± 0.9 | 1.7 ± 0.7 | 2.7 ± 0.2† |
| Promyelocytes, Myelocytes, % | 5.0 ± 1.0 | 5.5 ± 0.5 | 10.4 ± 1.0† | 7.4 ± 1.0 | 3.8 ± 0.8 | 2.7 ± 0.2† |
| Metamyelocytes, Neutrophils, % | 38.0 ± 1.0 | 13.5 ± 3.5* | 41.8 ± 6.4 | 21.2 ± 3.2* | 58.2 ± 2.8† | 26.8 ± 3.5* |
| Monocytes, % | 4.5 ± 0.5 | 9.5 ± 1.5 | 10.6 ± 1.2† | 10.2 ± 1.9 | 7.7 ± 1.5 | 9.5 ± 1.0 |
Peripheral blood and bone marrow from mBSA/IL-1-treated B6 and G-CSF-/- mice were analyzed on days 0 (baseline), 3, and 7. Data are representative of absolute numbers or % ± SEM; n > 4 mice per group. *, P < 0.05 between groups at time point examined. †, P < 0.05 between mice of same genotype compared with day 0.