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. 2004 Jul 22;101(31):11416–11421. doi: 10.1073/pnas.0403555101

Fig. 2.

Fig. 2.

Total, enriched, or highly purified CD11c+ and CD11c– cells from BM are activated in response to HSV-1 to produce IFN-α, even in the absence of TLR9. WT total BM cells [2.5 × 106 per ml, in the presence of IL-3 and GM-CSF (A)] or CD45RA-enriched [2.5 × 106 per ml (B)], B220-enriched [2.5 × 106 per ml (C)], or CD19-enriched [1 × 106 per ml (D)] WT BM cells were stimulated with HSV-1or CpG-2216. B220-enriched (E) or CD11c-enriched (F) BM cells (2.5 × 106 per ml) from WT or TLR9 KO mice were stimulated with HSV-1 or CpG-2216 in the presence of IL-3 and GM-CSF. Sorted CD11c+B220highCD45RAhigh pDC (G) or CD11c+B220–CD45RA– non-pDC (H) cells (2.5 × 105 per ml) were stimulated with either 107 pfu/ml [multiplicity of infection (MOI) 40] or 106 pfu/ml (MOI 4) of HSV-1 or CpG-2216, in the presence of IL-3 and GM-CSF. All supernatants were tested by ELISA for IFN-α production. One representative experiment of at least three is shown. Error bars represent the range of duplicate samples; asterisks indicate that production of IFN-α was below the detection limit of the ELISA.