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Pervanadate-induced tyrosine phosphorylation state of CD28 does not lead to movement to the detergent-insoluble fraction. Jurkat cells at 108 cells per ml in complete RPMI medium 1640 were treated with pervanadate or with hCD28 Ab or left unstimulated. After lysis in 1% Nonidet P-40, CD28 was immunoprecipitated from the detergent-soluble and -insoluble fractions and then analyzed by immunoblotting as indicated.
