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Stimulation of either hCD28 or hCD3 on hCD28+mCD28+ Jurkat cells fails to induce the translocation of mCD28 to the detergent-insoluble fraction. Jurkat cells expressing WT mouse CD28 (mCD28) and endogenous human CD28 (hCD28) were stimulated with an anti-mCD28 mAb, an anti-hCD28 mAb, or an anti-hCD3 or left unstimulated. The cells were lysed, and the detergent-insoluble fractions were analyzed for the presence of mCD28 by immunoprecipitation followed by immunoblotting.
