| Gene | Oligonucleotides | Sequence (5¹-3¹) | Amplicon size (bp) | Tₐ (°C) |
| Nanog | Forward primer Reverse primer | CCCAGCTGTGTGTACTCAAT GGTTCAGGATGTTGGAGAGTT | 87 | 60 |
| Oct 3/2 | Forward primer Reverse primer | GGGAGGAGCTAGGGAAAGA TCCTTCCTTAGTGAATGAAGAACT | 77 | 60 |
| Sox2 | Forward primer Reverse primer | TGCAGTACAACTCCATGACC GGACTTGACCACCGAACC | 125 | 55 |
| CD133 | Forward primer Reverse primer | CCAGAAGCCGGGTCATAAAT ATTCACTCAAGGCACCATCC | 127 | 56 |
| bp, base pairs of amplicon size; Tₐ, annealing temperature |
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