Fig. 1.

Effect of hTrx on postischemic myocardial apoptosis. (A) Representative photograph of electrophoretic analysis of internucleosomal DNA extracted from sham-operated control hearts (S) or mouse hearts exposed to I/R receiving either vehicle (V) or hTrx. (B) Representative photographs of TUNEL staining. Nuclei of normal cells are stained blue, and apoptotic nuclei are stained red. (C) Summary of TUNEL-positive myocytes from 8-10 mice per group. Assays were performed in a blinded fashion. (D) Summary of caspase-3 activity determined with a colorimetric assay kit. n = 10-12 per group. ^**, P < 0.01 vs. vehicle group. (E) Representative photographs of hTrx immunostaining confirming uptake of hTrx by cardiomyocytes.