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Coimmunoprecipitation of phyC-phyE with the myc6-phyB protein from seedling extracts. Samples of extracts of light- and dark-grown seedlings of the WT, phyB mutant, and phyB(mycPHYB) lines were immunoprecipitated with the anti-myc 9E10 mAb or with 1 μg of nonimmune mouse IgG. Aliquots of the input extracted proteins and the immunoprecipitates were separated on 6% SDS gels, blotted, and probed with the indicated antibodies (in, input to IP reaction).
