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SEC of phytochrome dimers. Extracts of WT Arabidopsis and phyB- (mycPHYB) seedlings were prepared and separated on Superose 6. (A) Profiles of scanned immunoblots for each of the five Arabidopsis phytochromes from SEC of the WT extract. Column fractions were separated on 6% SDS gels, blotted, and probed with the phy-specific mAb. (B) Coimmunoprecipitation of phyC-phyE with myc6-phyB from individual fractions from SEC of an extract of the phyB(mycPHYB) line.
