Table 2.
The Relative Solvent Accessibility of ABPαβγ ω+ Sites That Map to the Recombinant Cat Allergen Fel DI (PDB 1PUO)
|
1PUO chain 1
|
ABPα
|
1PUO chain 2
|
ABPβγ
|
|||
|---|---|---|---|---|---|---|
| All sites | Mouse and rat ω+ | Mouse only ω+ | All sites | Mouse and rat ω+ | Mouse only ω+ | |
| No. of residues | 68 | 4 | 5 | 67 | 29 | 17 |
| No. buried | 5 | 0 | 0 | 6 | 3 | 1 |
| No. intermediate | 22 | 0 | 0 | 19 | 6 | 2 |
| No. exposed | 41 | 4 | 5 | 42 | 20 | 14 |
| Buried (%) | 7.4 | 0.0 | 0.0 | 9.0 | 10.3 | 5.9 |
| Intermediate (%) | 32.4 | 0.0 | 0.0 | 28.4 | 20.7 | 11.8 |
| Exposed (%) | 60.3 | 100.0 | 100.0 | 62.7 | 69.0 | 82.4 |
The solvent accessibility values of cat Fel dl residues equivalent to ABPαβγ ω+ residues were calculated from the structure coordinates using the DSSP algorithm (Kabsch and Sander 1983). Relative accessibility scores were partitioned into three states: buried (<9% relative accessibility), intermediate (9%-35% relative accessibility), and exposed (≥36% relative accessibility), as described previously (Rost and Sander 1994).