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. 2004 Aug 17;2(8):e217. doi: 10.1371/journal.pbio.0020217

Figure 1. Comparison of Antisense and Inverted-Repeat Silencing of pmt .

Figure 1

Nicotine content (mean of 5–6 plants/line) normalized to mean of WT of unelicited (control) N. attenuata plants and plants 5 d after elicitation with 150 μg of MeJA per plant from independent lines transformed with (A) antisense pmt constructs and (B) an IRpmt construct. In contrast to the 31 lines transformed with the antisense pmt construct, 29 of the 34 IRpmt lines had dramatically reduced constitutive and MeJA-induced nicotine levels. T, terminator; P, promoter; I, spliceable intron; arrow, 950-bp consensus fragment of pmt1 and pmt2. For details of transformation constructs see Protocol S1.