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. 2004 Aug 17;2(8):e218. doi: 10.1371/journal.pbio.0020218

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Copyright: © 2004 Kumar et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Expression of Msx1 mRNA in active myofiber. Note the accumulation of reaction product around nuclear aggregates (arrowed).

(B) Absence of significant Msx1 mRNA expression in a quiescent fiber. This image is taken from the same culture as (A).

(C) Expression of NRad mRNA in nuclei of quiescent fibers (arrowed). Comparable intensity was observed for NRad expression by active fibers.

(D) Expression of Msx1 mRNA in nuclei of fibers (arrowed) made quiescent by culture in taxol. Note the difference in Msx1 expression levels between the taxol-induced inactive fibers and normal quiescent myofibers in (B). Scale bar, 50 μm.