Skip to main content
. 2004 Aug 17;2(8):e235. doi: 10.1371/journal.pbio.0020235

Figure 3. Activation of the HAC1 Promoter Controls Increase in HAC1 mRNA Abundance.

Figure 3

(A) Analysis of HAC1 promoter activity during bipartite stress conditions. Δhac1 cells containing either a construct restoring HAC1 expression (lanes 1–4) or a construct expressing GFP driven by the HAC1 promoter (lanes 5–8) were grown at 23 °C and shifted to 37 °C concurrent with addition of DTT as indicated.

(B) Determination of mRNA half-life during HAC1-mRNA-inducing conditions. polIIts cells were grown at 23 °C and were shifted to 37 °C either in the absence (open symbols) or presence (filled symbols) of DTT. HAC1 mRNA abundance (squares) and ACT1 mRNA abundance (circles) are normalized to the abundance of the PolIII transcript SCR1.