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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Cancer Res. 2016 Sep 20;76(21):6183–6192. doi: 10.1158/0008-5472.CAN-15-3125

Figure 4.

Figure 4

BPTF depletion sensitizes tumor cells to CD8 T cell cytotoxicity in vitro. A-D, percent target cell cytotoxicity determined by LDH release. A, purified splenic CD8 T cells from control or BPTF KD tumor bearing mice were cocultured on control or BPTF KD targets, respectively, at the indicated effector to target (E:T) ratios. (n = 3, * = ttest pvalue < 0.05). B, coculture of naïve purified CD8 T cells treated with PMA + Ionomycin with 4T1 or B16F10 targets at a 10:1 E:T ratio. C, CD8 T cell clones isolated from spleens of 4T1 control or BPTF KD tumor bearing mice were cocultured with 4T1 control or BPTF KD targets, respectively, at a 10:1 E:T ratio. Each dot represents one clone and is an average of 3 biological replicates (* = ttest pvalue < 5.0×10−3). D, six representative CD8 T cell clones from panel C were cocultured with either control or BPTF KD 4T1 targets at a 10:1 E:T ratio. Results are representative of 3 biological replicates for each clone (* = ttest pvalue < 0.04).