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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Cancer Res. 2016 Sep 20;76(21):6183–6192. doi: 10.1158/0008-5472.CAN-15-3125

Figure 5.

Figure 5

BPTF regulates immunoproteasome and TAP subunit expression. A, microarray heat map of genes significantly deregulated in BPTF KD 4T1 tumors from NSG mice (n = 3). Scale represents +/− 3 fold expression change. Genes related to the immune response are highlighted. B, qRT-PCR analysis of Psmb8, Psmb9, Tap1 and Tap2 expression from control or BPTF KD 4T1 and B16F10 cells. (n = 3 biological replicates, * = ttest pvalue < 0.05). C, PSMB8, PSMB9, TAP1 and TAP2 Western blot analysis of 4T1 (top) and B16F10 (bottom) total cell extracts. D, representative flow cytometry histograms of 4T1 and B16F10 cells stained for the MHC class I molecules H2K and H2D. E, fold change in MFI of H2K and H2D (n ≥ 3 biological replicates, * = ttest pvalue < 0.05). F, qRT-PCR analysis of MHC class I gene expression from 4T1 and B16F10 cells (n = 3 biological replicates).