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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Cancer Res. 2016 Aug 30;76(21):6362–6373. doi: 10.1158/0008-5472.CAN-16-1306

Figure 4. H3K27me3 demethylases UTX and Jmjd3 regulated DPYD expression.

Figure 4

A. Correlations between DPYD and UTX (left panel) or JMJD3 (right panel) expression were determined using normalized RNA read counts. B. Whole cell lysates from HCT116 and Hela cells treated with the indicated concentrations of GSK-J4 were immunoblotted using the indicated antibodies. C. H3K27me3 was immunoprecipitated from HCT116 cells treated with 1 µM GSK-J4 or DMSO control and analyzed by qPCR. D. Expression of DPYD was measured in cells from (B). E. The viability of HCT116 cultures treated with 5-FU, GSK-J4, 5-FU/GSK-J4 combination, or non-treated control (NT) was measured by realtime cell analysis. In all panels means +/− SD are represented by bars and whiskers. * p<0.05; ** p<0.01; *** p<0.001; **** p<0.0001