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. Author manuscript; available in PMC: 2016 Nov 6.
Published in final edited form as: Leukemia. 2016 May 6;30(11):2221–2231. doi: 10.1038/leu.2016.107

Figure 5. Reversal of DNA damage and apoptosis of irradiated FDC-P1 cells in vitro by exposure to murine MSC-EVs.

Figure 5

A, B: Phosphorylated H2AX and cleaved PARP levels in FDC-P1 cell. (A) Cells were harvested at 1, 4, 18 and 24 hours after 500 cGy irradiation. (B) Cells were harvested at 1 and 4 after 1000 cGy irradiation. Protein expression was assayed by Western blot analysis. (C) 500 cGy irradiated FDC-P1 cells were treated with 2×109/ml of murine MSC-EVs for 18 hours. Phosphorylated H2AX and cleaved PARP were measured by Western blot analysis. Actin or GAPDH was used as an internal control. The experiments were repeated three times. (D) DNA fragmentation in FDC-P1 cells 18 hours-post 500 cGy irradiation with murine MSC-EVs treatment was evaluated using a Cell Death Detection ELISA kit. The data are expressed as mean ± SEM of three separate experiments. *, P<0.05 compared to control cells, **, p<0.05 compared to 500 cGy irradiated group.