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. 2004 Aug 17;2(8):e246. doi: 10.1371/journal.pbio.0020246

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Copyright: © 2004 Patil et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Gcn4p levels, but not eIF-2α phosphorylation, rise under ER stress in a UPR-dependent manner. Cells bearing a C-terminally myc-tagged allele of GCN4 were treated with Tm for 0, 15, 30, 60, or 120 min. Western blots probed with anti-myc recognizing Gcn4p-myc (top panels) or phospho-specific anti-eIF-2α antibody (bottom panel). Gcn4p blot for the Δgcn2 mutant is 5x overexposed so that the bands are visible.

(B) Quantitation of the Gcn4p-myc protein levels in Figure 4A. Data reflect an average of four experiments, normalized against Gcn4p levels in the WT t = 0 samples.