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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Cancer Res. 2016 Aug 30;76(21):6290–6298. doi: 10.1158/0008-5472.CAN-16-0087

Figure 5. Control of expression of MNX1 by AKT.

Figure 5

A. LNCaP cells were treated with 500nM AZD5363 for 24 hrs and cell lysates prepared. Western blot for MNX1, P-AKT (S473) and P-S6 were performed. Actin is a loading control. Note increased AKT phosphorylation is due to feedback induced by inhibition of AKT kinase activity.

B. LNCaP cells were treated with either 20 uM LY294002 or 1uM rapamycin or vehicle only for 24 hrs and cell lysates prepared. Western blot for MNX1, P-AKT (S473) and P-S6 were performed. Actin is a loading control.