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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Cancer Prev Res (Phila). 2016 Sep 13;9(11):875–886. doi: 10.1158/1940-6207.CAPR-16-0161

Figure 4. Inhibition of α7-nAChR/PI3K signaling contributes to cell migration suppression by BCX.

Figure 4

BEAS-2B, A549, MDA-MB-231 and 293T cells were treated with BCX (0.5–4.0 μM) or MG624 (1.0 μM) in the presence of nicotine (1.0 μM) or PNU282987 (1.0 μM) for 12 hr. The migrated cells were quantified with nicotine (A) or PNU282987 (B) treatment. The inhibition of α7-nAChR agonists-induced cell migration by BCX in BEAS-2B (C) or A549 cells (D) was calculated respectively and compared with blank without stimulation. BEAS-2B, U87MG or MCF-7 cells were treated with BCX (0.5–4.0 μM) or MG624 (1.0 μM) for 12 hr, Cell migration was analyzed treated with overexpression of constitutively active PI3K or mutant of PTEN (E). BEAS-2B and A549 cells were treated with BCX (0.5–4.0 μM) or MG624 (1.0 μM) for 48 hr, then the MMP2 level was measured without PNU282987 treatment (F) or treated with 1.0 μM PNU282987 for 1 hr (G).