Figure 2.

Alternative pathway-mediated complement amplification on A. fumigatus. Complement activation was measured on A. fumigatus (1 × 10⁷ conidia/ml) after incubation in various buffers and sera. (A,B) NHS-generated C3b and TCC with/without Mg²⁺ in the dilution buffer. (C,D) C3b and TCC from C2-deficient serum with/without reconstitution of C2 (E,F) NHS-generated C3b and TCC under Mg²⁺/EGTA and EDTA conditions. Complement products were measured by flow cytometry and expressed as mean fluorescence intensity (MFI). Results represent the means of three independent experiments ± SD, *p ≤ 0.05, **p ≤ 0.01 (two-tailed paired Student’s t-test or one-way ANOVA, Bonferroni’s multiple comparison test).