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Reconstitution of C1q- and MBL-deficient sera. A. fumigatus (1 × 107 conidia/ml) was added into (A–C) C1q- or (D–F) MBL-deficient serum diluted in TBS/Ca2+ (no Mg2+). Complement products were measured by flow cytometry and expressed as mean fluorescence intensity (MFI): (A,D) C4b, (B,E) C3b, and (C,F) TCC. Results represent the means of three independent experiments ± SD, *p ≤ 0.05, ****p ≤ 0.0001 (two-tailed paired Student’s t-test).
