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. 2016 Nov 3;7:473. doi: 10.3389/fimmu.2016.00473

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Copyright © 2016 Rosbjerg, Genster, Pilely, Skjoedt, Stahl and Garred.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MBL-mediated opsonophagocytosis of A. fumigatus in C1q-deficient serum. FITC-conjugated A. fumigatus conidia (1 × 10⁷ conidia/ml) were opsonized with C1q-deficient serum including MBL inhibitor or MBL mock-inhibitor. Opsonized conidia were mixed with isolated human neutrophils in a ratio of 5:1, and phagocytosis was quantified by flow cytometry. (A) The percentage of phagocytizing neutrophils. (B) Phagocytic index (percentage of phagocytizing neutrophils × MFI). (C) Fluorescence and DIC microscopy image of neutrophils phagocytizing A. fumigatus-FITC. Results represent the means of three independent experiments ± SD, *p ≤ 0.05 (two-tailed Student’s t-test). MFI = mean fluorescence intensity.