Figure 7.
MBL-mediated complement activation in the absence of IgM binding. NHS and UCS pools with “high” and “low” contents of MBL were mixed with A. fumigatus (1 × 107 conidia/ml) to determine the following components: (A,B) IgG binding, (C,D) IgM binding, (E) C3b generated from “high-MBL” NHS and “high-MBL” UCS, and (F) C3b generated from “low-MBL” UCS and “low-MBL” NHS. Deposited C3b and bound IgG/IgM were measured by flow cytometry and expressed as mean fluorescence intensity (MFI). Results represent the means of three independent experiments ± SD, ****p ≤ 0.0001 (unpaired Student’s t-test).