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Mass spectra of ginkgotides gB5 from LC-ESI-LTQ-Orbitrap MS/MS in positive ion mode. The purified gB5 was S-reduced by 20 mM dithiothreitol, S-alkylated with 200 mM iodoacetic acid and subsequently de-salted by C18 Ziptip. The spectrum was scanned between mass ranges of (A) 100 and 2000 m/z, (B) 100 and 900 m/z, and (C) 800 and 1800 m/z. Assignment of isobaric amino acids such as Leu/Ile were confirmed by the transcriptome.
