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. 2016 Nov 3;10:494. doi: 10.3389/fnins.2016.00494

Figure 4.

Figure 4

Spectrin breakdown product (SBDP) 145/150 is elevated in young DBA/2J ON and SC while β-tubulin is decreased in a transport-dependent manner in pathologically-aged ON. (A) Chemiluminescence-based quantification using ProteinSimple Wes technology illustrates significantly higher levels of SBDP(145/150) within ON of D3-5 mice compared to all other groups, with no differences seen in pathologically-aged (10–12 months.) DBA/2J mice regardless of transport outcome. Bar graph (top) shows SBDP145/150 and β-tubulin normalized to GAPDH (a housekeeping gene marker), while corresponding examples of automated western output are shown below for ON. Importantly, a significant decrease in β-tubulin was observed in pathologically-aged ON associated with zero anterograde transport to the corresponding SC compared to controls and D10-12 mice with intact transport. (B) A significant elevation in SBDP chemiluminescence was observed in D3-5 SC compared to D2G controls (B), with no differences in collicular β-tubulin elucidated. Bar graph (top) shows SBDP145/150 and β-tubulin normalized to GAPDH, while corresponding examples of automated western output are shown below for SC. (Refer back to Figure 1 to see β-tubulin loss using immunofluorescence.) Error bars depict SEM. Asterisk indicates that groups are significantly different from bracketed comparison (p <0.05)