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. 2016 Nov 3;4:120. doi: 10.3389/fcell.2016.00120

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Copyright © 2016 Clark and Petty.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Calibration of an EMCCD Camera. It is important to calibrate the camera output to insure that its performance is linear throughout the brightness region studied in tissue experiments. As described in the text, individual beads were photographed. The images were then analyzed to determine the average intensity of each bead preparation. The average bead intensity is plotted at the ordinate and the percentage of maximal label, as provided by the manufacturer, is plotted at the abscissa. Note that the output of the camera is linear with the extent of label.