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. 2016 Nov 3;4:120. doi: 10.3389/fcell.2016.00120

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Copyright © 2016 Clark and Petty.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pseudocolor BRIM images of CD44^hi/CD24^lo cells and N-cad^hi/E-cad^lo cells of DCIS lesions are shown. (A,C) show CD44^hi/CD24^lo cells whereas (B,D) show N-cad^hi/E-cad^lo cells. Positive BRIM findings (high ratios) are shown in (A,B). Negative BRIM results (low ratios) are shown in (C,D). Higher ratios are indicated by the red-yellow colors in (A,B). Arrows denote regions of higher ratios. Note that the CD44^hi/CD24^lo cells are primarily found at the periphery of the ducts in (A). These data show that patients can differ widely in the ratios associated with DCIS. (Ratio scale bars are given along the right hand side of each panel) (20x objective).