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. 2016 Nov 3;7:1755. doi: 10.3389/fmicb.2016.01755

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Copyright © 2016 Wang, Li, Lin, Pang, Liu, Liu, Wang, Zhang, Wu, Lin and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confirmation of rsrR and rsrS mutants by PCR analyses. (A,B) Diagram of three sets of primers specific to target genes (rsrR and rsrS), the upstream and downstream homologous arms (UHA and DHA) and the sequences outside of homologous arms, respectively. (C,D) PCR analyses of the chromosomes of ΔrsrR and ΔrsrS. Lane 1 and 2, PCR amplifications from wild type and mutants using primers R5F/R or S5F/R, respectively; lane 3 and 4, PCR amplifications from wild type and mutants using primers R4F/R or S4F/R, respectively; lane 5 and 6, PCR amplifications from wild type and mutants using primers R3F/R or S3F/R, respectively. In (C,D), the arrows are added to indicate the target bands.