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. 2016 Nov 3;7:1755. doi: 10.3389/fmicb.2016.01755

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Copyright © 2016 Wang, Li, Lin, Pang, Liu, Liu, Wang, Zhang, Wu, Lin and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The functional identification of a 19-bp inverted repeat sequence (IRS) upstream the tetH promoter. (A) The loci of IRS, −10/−35 regions, the transcriptional start site and primers on the sequence upstream tetH gene. (B–D) The results of EMSAs to determine the binding ability of RsrR to the regulatory sequence. −, the group containing the nucleotide fragments without RsrR; +, the group containing both the different nucleotide fragments and RsrR. G360, a 360 bp-fragment from the gapdH gene used as a control; T360, T148 and T90, a 360 bp-, 148 bp-, or 90 bp-fragment amplified from upstream region of tetH; G360+58IRS, a 58 bp sequence containing the IRS fused with the 360 bp-fragment from gapdH; T360Δ19: the T360 fragment with IRS removed. (E) Diagram of series of IRS-probe vectors containing various versions of the upstream region of tetH. (F) Transcriptional analysis of gusA on the IRS-probe vectors in ΔrsrR and the wild type.