Table 1.
Strains and plasmids used in the study.
| Strains or plasmids | Genotype or description | Source or references |
|---|---|---|
| STRAINS | ||
| A. caldus MTH-04 | Isolated from Tengchong area, Yunnan province, China | Our lab |
| A. caldus MTH-04 ΔrsrR mutant | ΔrsrR | This study |
| A. caldus MTH-04 ΔrsrS mutant | ΔrsrS | This study |
| Escherichia coli DH5α | 80d lacZΔM15Δ(lacZYA-argF) U169 end A1 recA1 hsdR17(rk−,mk+) supE44λ-thi-1 gyr96 relA1 phoA | TransGen Biotech Corp. China |
| Escherichia coli SM10 | Thr leu hsd recA KmrRP4-2-Tc::Mu | Simon et al., 1983 |
| Escherichia coli BL21 (DE3) | F−ompT hdsSB(Rb−mB−) gal dgmmet(DE3) | TransGen Biotech Corp. China |
| PLASMIDS | ||
| pMD19-T | Apr, ColE1 replicon | TaKaRa Cor. |
| pSDUDI | suicide plasmid; Apr; Kmr; oriTRP4; multi-cloning sites | This study |
| pSDUDI::rsrR(UHA + DHA) | suicide plasmid for rsrR deletion | This study |
| pSDUDI::rsrS(UHA + DHA) | suicide plasmid for rsrS deletion | This study |
| pSDU1-tac | Cmr; IncQ, mob+, tac promoter | Our lab |
| pSDU1-I-SceI | Cmr; mob+; Ptac; containing I-SceI gene | This study |
| pET-22b | Ampr | Novagen Cor. |
| pJRD215 | Smr, Kmr; IncQ, Mob+ | Davison et al., 1987 |
| pJRD-P360IRS | Smr, Kmr; IncQ, Mob+; PtetH(360 bp); gusA | This study |
| pJRD-P148IRS | Smr, Kmr; IncQ, Mob+; PtetH(148 bp); gusA | This study |
| pJRD-P90 | Smr, Kmr; IncQ, Mob+; PtetH(90 bp); gusA | This study |
| RP4 | Apr, Tcr, Kmr; IncP, tra+ | Datta et al., 1971 |
| pACBSR | Cmr; I-Sce I, λ-Red recombination system | Herring et al., 2003 |