Table 2.

Primers used in construction of suicide plasmid and I-Sce I-expressing plasmid.

Primer name	Primer Sequence (5′ → 3′)
oriT EcoR sen	ATTCCGGAATTCGCTCGTCCTGCTTCTCTTCG
oriT Sal ant	TCACGCGTCGACCGGGATTCAACCCACTCG
pMD19 Sal sen	TCACGCGTCGACGCGGTAATACGGTTATCCACAG
pMD19 EcoR ant	ATTCCGGAATTCAATGGTTTCTTAGACGTCAGGT
pMD19 Nde sen	TTATCATATGCAATTGAAGCTTGGTACCGCGGCTAGCGG
	CCGCGCGGTAATACGGTTATCCACAG
oriT Apa ant	TATAGGGCCCCGACCGGGATTCAACCCA
Km Apa sen	GTGAGGGCCCATAGGGATAACAGGGTAATAT GAATGTCA
	GCTACTGG
Km Nde ant	TTATCATATGACGCGTGGATCCGAGCTCTAGACTAG
	TCGACAATCGAAATCTCGTGATGG
pSDU1 Xba sen	CTTCATGCATTCTAGATCATGTTTGACAGCTTATC
tac Bam ant	TCGCGGATCCTCCTGCAGTGTTTCCTGTGTGAAATTG
I-Sce Bam sen	TTACGCGGATCCAGGAGGGTACCTATATGCATATGAAAAA
	CATCAAAAAAAACC
I-Sce Xba ant	TTCTTCTCTAGAACGTCGGGCCCTTATTTCAGGAAAGTTT
	CGGAGGAG

Restriction sites were indicated with underline.