. 2016 May 28;8(4):262–274. doi: 10.1007/s12560-016-9246-4

Table 2.

Primer sets and amplicon sizes for the detection of HAdV species and serotypes

Species	Serotypes	Primer	Sequence (5′–3′)	Amplicon size (bp)	Target region	References
B	Ad3	Ad3F	GGTAGAGATGCTGTTGCAGGA	503	Ad3 hexon	Metzgar et al. (2005)
B	Ad3	Ad3R	CCCATCCATTAGTGTCATCGGT	503	Ad3 hexon	Metzgar et al. (2005)
	Ad7	Ad7F	GGAAAGACATTACTGCAGACA	311	Ad7 hexon
	Ad7	Ad7R	AATTTCAGGCGAAAAAGCGTCA	311	Ad7 hexon
	Ad21	Ad21F	GAAATTACAGACGGCGAAGCC	237	Ad21 hexon
	Ad21	Ad21R	AACCTGCTGGTTTTGCGGTTG	237	Ad21 hexon
C		AdCF	TGCTTGCGCTHAAAATGGGCA		AdC fibre	Adhikary et al. (2004)
	Ad1	Ad1R	CGAGTATAAGACGCCTATTTACA	630	Ad1 fibre
	Ad2	Ad2R	CGCTAAGAGCGCCGCTAGTA	204	Ad2 fibre
	Ad5	Ad5R	ATGCAAAGGAGCCCCGTAC	455	Ad5 fibre
	Ad6	Ad6R	CTTGCAGTCTTTATCTGAAGCA	929	Ad6 fibre
E	Ad4	Adeno4.U3	CAAGGACTACCAGGCCGTCA	254	4 hexon	Hough et al. (2002)
E	Ad4	Adeno4.L1	TTAGCATAGAGCATGTTCTGGC	254	4 hexon	Hough et al. (2002)
F		AdF1	ACTTAATGCTGACACGGGCAC		AdF fibre	Xu et al. (2000)
	Ad40	K402	CACTTAATGCTGACACG	152	Long fibre gene
	Ad41	K403	ACTGGATAGAGCTAGCG