Fig. 5.

Regulation of chemotaxis by BCR activation. Boyden chamber migration assays were performed towards CXCL12 (100 ng/ml; n = 8) and CCL21 (200 ng/ml; n = 7) after 24-h stimulation with α-IgM, α-IgD, or both (10 μg/ml). Cells were allowed to migrate for 2 h and subsequently stained for CD19/CD5 and for viability using Annexin-V-FITC and 7AAD. Only Annexin/7AAD double-negative and CD19/CD5 double-positive cells were counted. All experiments were performed as duplicates; results are shown as the percentage of input cells