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Impact of kinase inhibitors on BCR- and chemokine-mediated calcium mobilisation. Calcium mobilisation of CLL cells in response to α-IgM (10 μg/ml), α-IgD (10 μg/ml), CXCL13 (500 ng/ml), CCL21 (200 ng/ml), and CCL19 (200 ng/ml) was assessed as described. After loading with Fluo-3-AM, cells were additionally incubated for 30 min with 5 μM Bafetinib, Ibrutinib, R406, or an equal volume DMSO as a control (n = 11)
