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. 2016 Apr 7;44(11):3319–3334. doi: 10.1007/s10439-016-1601-y

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Cell distribution studies and quantification of the positive cells via confocal microscopy. CD68 (green) and CD163 (red) immunofluorescence staining of macrophage and fibroblast mono-cultures and fibroblast-macrophage juxtacrine (FM Juxtacrine CC) and paracrine co-cultures (FM Paracrine CC) in (a) TPU and (b) ePTFE grafts after 21 days. The nuclei of cells were counterstained with DAPI (blue). (c) Quantification of the CD68 and CD163 positive cells on TPU and ePTFE grafts after 21 days in macrophage (M) and fibroblast (F) mono-cultures and fibroblast-macrophage juxtacrine (FM JUX CC) and paracrine co-cultures (FM PAR CC). Data are presented as mean ± standard deviation (n = 3 per group).