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. 2016 Oct 25;7:13172. doi: 10.1038/ncomms13172

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Copyright © 2016, The Author(s)

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(a) MTSD versus elongation correlations are indistinguishable in the embryonic epidermis (St 12—St 15, blue, both graphs), pupal wing epithelium (24APF, red, both graphs) and in silico cells (F, black, bottom only). In the follicular epithelium (St 7—St12 egg chambers, green, both graphs), although the slope is retained, the correlation is shifted towards larger MTSD values. Bottom graph shows top graph data plus in silico cells. (b) Representative images of pupal wing epithelium 24APF, visualized as described in Fig. 1, from wild-type (left), pupal wings from transheterozygous ds^38k/ds^UA071 mutants (centre) and pupal wings from dpy^ov1 mutants (right). Scale bars, 10 μm. (c) MTSD versus eccentricity correlation is retained between wild-type (blue), ds^38k/ds^UA071 (orange) and dpy^ov1 (brown) 24APF pupal wing epithelium cells. (d) Quantification of cell elongation (eccentricity and cell's long/short axes ratios; left), MTSD (centre) and MTDEV (right) shows reduced cell elongation and MT organization in dpy^ov1 pupal wings. Cells from 7 pupal wings (three independent experiments) were analysed. Statistical analysis was performed with Mann–Whitney test (eccentricity) and unpaired t-test (MTSD and MTDEV). Asterisks represent statistical significance P**<0.01. Each dot in a and c represents the mean MTSD value (mean±s.e.m.) of experimental or simulated cells with corresponding eccentricities±0.01. Cell number and corresponding eccentricity (parenthesis) were in a: for embryonic epidermis: 100 (0.7), 138 (0.75), 182 (0.8), 245 (0.85), 334 (0.9), 520 (0.95) from 42 wild-type embryos; for pupal wing epithelium: 35 (0.7), 35 (0.75), 45 (0.8), 43 (0.85), 46 (0.9), 35 (0.95) from 4 wild-type pupae; for follicular epithelium: 31 (0.7), 15 (0.75), 11 (0.8), 19 (0.85), 16 (0.9), 13 (0.95) from 6 wild-type female flies. In d, for wild-type: 22 (0.7), 26 (0.75), 42 (0.8), 60 (0.85), 94 (0.9), 109 (0.95) from 7 pupae; for dpy^ov1: 62 (0.7), 82 (0.75), 61 (0.8), 51 (0.85), 43 (0.9), 9 (0.95) from 7 pupae; for ds^38k/ds^UA071: 24 (0.7), 32 (0.75), 54 (0.8), 45 (0.85), 37 (0.9), 9 (0.95) from 4 pupae.