Supplementary Figure 5.

CCR4⁺ T_CM resemble Th₂-like cells in respect to their expression of Th₁ associated genes. PBMC from healthy donors, as well as cells isolated before or after 1 or 12 months of ART from HIV-1-infected adults were stained with the “sorting” panel (Supplementary Table 1). Subsets of CD4⁺ T cells were sorted as indicated in Supplementary Figure 4 and their gene expression profiles determined by multi-parametric quantitative RT-PCR. (A) Th₁-associated genes were compared in Th_2-like, CCR4⁺ T_CM, and Th₁-like cells isolated from healthy donors. (B) CCR4⁺ T_CM from HIV-1⁺ patients after 1 month of ART were compared to their counterparts from healthy donors in respect to expression of Th₁-associated genes. Bar graphs show interquartile ranges, median bars, as well as individual data points. Statistically significant differences are indicated: * P < 0.01, ** P < 0.001, *** P < 0.0001.