Table 1. Characteristics of synthetic HA derivatives.
| HA derivative | HA | ATTO565- HA | sHA1 | ATTO565-sHA1 | ATTO565- ATTO655-sHA1 |
|---|---|---|---|---|---|
| D.S. | — | — | 1.5 | 1.0 | 1.4 |
| fluorescent label [μg/mg] | — | 0.47 | — | 1.40 | ATTO655: 0.33 ATTO565: 2.89 |
| Mw [g/mol] | 84,000 | 27,000 | 42,000 | 27,000 | 27,000 |
| PD | 2.5 | 2.4 | 2.3 | 2.2 | 1.9 |
Degree of sulfation, number of sulfate residues per disaccharide unit (D.S.); weight-average (Mw) molecular weight values as determined by gel permeation chromatography equipped with Laser Light Scattering (LLS) detection; molecular weight distributions (polydispersity index: PD) based on the values calculated from RI detection.