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. 2016 Nov 3;6:36133. doi: 10.1038/srep36133

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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(A) NKX2-6 mRNA expression of c.1A > T (Mut) compared with wild-type (WT). mRNA levels normalized to that of GAPDH and then to WT. (B,C) Western blots showing the proteins produced by cells transfected with wild-type, c.1A > T, and c.A1_A135del (Trunc) NKX2-6 vector. Tubullin was used as the loading control for normalization.