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. 2016 Nov 3;6:36133. doi: 10.1038/srep36133

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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(A) Intracellular levels of wild-type (WT) and R551Q mutant (Mut) NRG1 proteins. GAPDH was used as the loading control for normalization. (B) Western blot analysis of the release of the extracellular domain in the culture medium. (C) Cellular localization of wild-type and mutants NRG1 was analyzed by immunofluorescence staining.