Figure 5. Phylogenetic tree of MaAmyB plus its 164 homologs, and a selection of members from the defined GH13 subfamilies that are either associated with EC3.2.1.1 or EC3.2.1.98² (see Supplementary Table S1).

The tree is based on the alignment of the catalytic AB-regions (GH13.hmm) as obtained from dbCAN. Domain organization shown is based on a combination of CDD and dbCAN data. The inserts in the A-region, the B-region and aberrant C-region as indicated are based on BLAST results of the identified regions from MaAmyB. Subfamily information was obtained from CAZy. Aberrant C-region information was obtained from Supplementary Fig. S2. The number 70 shows the location of a cluster of 70 putative α-amylases that share the aberrant C-region with the MaAmyB homologs but do not belong to subfamily GH13_42 (designated as GH13_VV) (see Supplementary Figs S2 and S3). At numbers 24 and 63, clades with these numbers of sequences similar to the nearby shown sequences have been collapsed to improve the readability of the tree. CBM74 is a novel CBM domain in MaAmyA¹⁵.