Figure 7.

(A) Protein domain organization of MaAmyA and MaAmyB and 4 related proteins. All proteins are shown with the N-terminus on the left hand side. The dotted line box indicates a stretch of domains that is highly similar between MaAmyA and MaAmyB (>99% identity), suggesting a relatively recent duplication event. Equal fill patterns indicate domains with high similarity. Solid filled domains do not have similarity to any other domain shown. AmlB (CAB06622.1) and BAA22082.1 are included as representatives of GH13_32 with high similarity to MaAmyA. AmlC (CAB06816.1) is included as a representative of GH13_42 with high similarity to MaAmyB. BAB86376.1 is included as a representative of a group of chitinases containing FNIII domains with high similarity to the 3 C-terminal FNIII domains of MaAmyA and all FNIII domains of MaAmyB. CBM74 is a novel CBM domain¹⁵. (B) Schematic representation showing the orientations of the genes encoding the M. aurum B8.A (MaAmyA, MaAmyB) and S. lividans TK24 (AmlB, AmlC) α-amylases.